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COMU: scope and limitations of the latest innovation in peptide acyl transfer reagents
Author(s) -
SubirósFunosas Ramon,
NietoRodriguez Lidia,
Jensen Knud J.,
Albericio Fernando
Publication year - 2013
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.2517
Subject(s) - scope (computer science) , reagent , computer science , combinatorial chemistry , chemistry , nanotechnology , materials science , organic chemistry , programming language
The methodology for peptide bond formation is undergoing a continuous evolution where the main actors are being renewed. In recent years, coupling reagents based on the Oxyma scaffold, such as the uronium salt COMU, has been a groundbreaking contribution to the field. The advantages of COMU over classic benzotriazole‐based reagents (HATU, HBTU, HCTU, TBTU) were proven in terms of solubility and coupling efficiency in bulky junctions in our groups and others. However, some aspects of the use of COMU need to be revised and improved, such as the stability of commercial samples in organic solvents, which hampers the compatibility with long synthesis in automated synthesizers. In this review, an overview of the main features and suggestions to improve the use of COMU are presented, along with a discussion on the best conditions for its use in microwave‐assisted peptide robots. Copyright © 2013 European Peptide Society and John Wiley & Sons, Ltd.