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A general synthetic method toward uridylylated picornavirus VPg proteins
Author(s) -
Heden van Noort Gerbrand J.,
Schein Catherine H.,
Overkleeft Herman S.,
Marel Gijsbert A.,
Filippov Dmitri V.
Publication year - 2013
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.2508
Subject(s) - picornavirus , nucleoprotein , genome , residue (chemistry) , computational biology , rna , virology , tyrosine , biology , chemistry , microbiology and biotechnology , virus , biochemistry , gene
Small proteins called viral protein genome‐linked (VPg), attached to the 5′‐end of the viral RNA genome are found as common structure in the large family of picornaviruses. The replication of these viruses is primed by this VPg protein linked to a single uridylyl residue. We report a general procedure to obtain such nucleoproteins employing a pre‐uridylylated tyrosine building block in an on‐line solid phase‐based approach. Copyright © 2013 European Peptide Society and John Wiley & Sons, Ltd.