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The crystal structure of samarosporin I at atomic resolution
Author(s) -
Gessmann Renate,
Axford Danny,
Evans Gwyndaf,
Brückner Hans,
Petratos Kyriacos
Publication year - 2012
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.2454
Subject(s) - antiparallel (mathematics) , crystal structure , monoclinic crystal system , chemistry , crystallography , peptide , bent molecular geometry , subfamily , molecule , stereochemistry , physics , biochemistry , organic chemistry , quantum mechanics , magnetic field , gene
The atomic resolution structures of samarosporin I have been determined at 100 and 293 K. This is the first crystal structure of a natural 15‐residue peptaibol. The amino acid sequence in samarosporin I is identical to emerimicin IV and stilbellin I. Samarosporin is a peptide antibiotic produced by the ascomycetous fungus Samarospora rostrup and belongs to peptaibol subfamily 2. The structures at both temperatures are very similar to each other adopting mainly a 3 10 ‐helical and a minor fraction of α‐helical conformation. The helices are significantly bent and packed in an antiparallel fashion in the centered monoclinic lattice leaving among them an approximately 10‐Å channel extending along the crystallographic twofold axis. Only two ordered water molecules per peptide molecule were located in the channel. Comparisons have been carried out with crystal structures of subfamily 2 16‐residue peptaibols antiamoebin and cephaibols. The repercussion of the structural analysis of samarosporin on membrane function is discussed. Copyright © 2012 European Peptide Society and John Wiley & Sons, Ltd.