Premium
TectoRNP: self‐assembling RNAs with peptide recognition motifs as templates for chemical peptide ligation
Author(s) -
Yamashita Kohei,
Tanaka Takahiro,
Furuta Hiroyuki,
Ikawa Yoshiya
Publication year - 2012
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.2444
Subject(s) - template , peptide , rna , ligation , chemical ligation , native chemical ligation , chemistry , combinatorial chemistry , dimer , biochemistry , biophysics , computational biology , nanotechnology , microbiology and biotechnology , biology , chemical synthesis , materials science , organic chemistry , in vitro , gene
TectoRNA, an artificial RNA with self‐assembling ability, has been employed as a structural platform for RNA nanotechnology and RNA synthetic biology. In this study, tectoRNA was applied as a specific template for chemical peptide ligation. On the basis of a self‐assembling tectoRNA, we designed and constructed a template RNA that facilitates peptide ligation depending on controlled dimer formation. Two RNA‐binding peptides were recognized by two peptide‐binding RNA motifs embedded in the template RNA, and chemical ligation was promoted because of the entropic effect of Mg 2+ ‐dependent dimerization. In a series of biochemical analyses, we determined the relationship between the structures of the tectoRNA‐based templates and the extent of acceleration in peptide ligation. Copyright © 2012 European Peptide Society and John Wiley & Sons, Ltd.