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Conventional and microwave‐assisted SPPS approach: a comparative synthesis of PTHrP(1–34)NH 2
Author(s) -
Rizzolo Fabio,
Testa Chiara,
Lambardi Duccio,
Chorev Michael,
Chelli Mario,
Rovero Paolo,
Papini Anna Maria
Publication year - 2011
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.1395
Subject(s) - peptide , peptide synthesis , chemistry , yield (engineering) , combinatorial chemistry , microwave irradiation , posttranslational modification , biochemistry , materials science , catalysis , enzyme , metallurgy
Attracted by the possibility to optimize time and yield of the synthesis of difficult peptide sequences by MW irradiation, we compared Fmoc/tBu MW‐assisted SPPS of 1–34 N ‐terminal fragment of parathyroid hormone‐related peptide (PTHrP) with its conventional SPPS carried out at RT. MWs were applied in both coupling and deprotection steps of SPPS protocol. During the stepwise elongation of the resin‐bound peptide, monitoring was conducted by performing MW‐assisted mini‐cleavages and analyzing them by UPLC‐ESI‐MS. Identification of some deletion sequences was helpful to recognize critical couplings and as such helped to guide the introduction of MW irradiations to these stages. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd.