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Derivatization of peptides as quaternary ammonium salts for sensitive detection by ESI‐MS
Author(s) -
Cydzik Marzena,
Rudowska Magdalena,
Stefanowicz Piotr,
Szewczuk Zbigniew
Publication year - 2011
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.1342
Subject(s) - chemistry , ammonium , dabco , peptide , derivatization , combinatorial chemistry , protein quaternary structure , tandem mass spectrometry , octane , tandem , peptide synthesis , quaternary , solid phase synthesis , chromatography , mass spectrometry , organic chemistry , biochemistry , materials science , protein subunit , composite material , gene , paleontology , biology
Abstract A series of model peptides in the form of quaternary ammonium salts at the N ‐terminus was efficiently prepared by the solid‐phase synthesis. Tandem mass spectrometric analysis of the peptide quaternary ammonium derivatives was shown to provide sequence confirmation and enhanced detection. We designed the 2‐(1,4‐diazabicyclo[2.2.2] octylammonium)acetyl quaternary ammonium group which does not suffer from neutral losses during MS/MS experiments. The presented quaternization of 1,4‐diazabicyclo[2.2.2]octane (DABCO) by iodoacetylated peptides is relatively easy and compatible with standard solid‐phase peptide synthesis. This methodology offers a novel sensitive approach to analyze peptides and other compounds. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd.