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Synthesis and self‐assembly of a neoglycopeptide: morphological studies and ultrasound‐mediated DNA encapsulation
Author(s) -
Gour Nidhi,
Mondal Sudipta,
Verma Sandeep
Publication year - 2011
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.1334
Subject(s) - supramolecular chemistry , self assembly , peptide , concanavalin a , mannose , hydrogen bond , dna , chemistry , covalent bond , aqueous solution , hydrophobic effect , biophysics , nanotechnology , combinatorial chemistry , biochemistry , materials science , biology , organic chemistry , molecule , in vitro
Self‐assembly in peptides and proteins is an often encountered concept, where constituent building blocks are recruited and stabilized, via carefully orchestrated hydrophobic interactions, hydrogen bonding and other non‐covalent interactions, to eventually reveal an array of supramolecular aggregates, with defined structural features. This study presents synthesis and self‐assembly of a mannosylated peptide in aqueous medium. Turbidimetric assay with Concanavalin A (Con A), a mannose binding protein, was conducted to confirm the presence of hydrophilic mannose group on the exterior surface of self‐assembled structures. DNA encapsulation in these soft structures was achieved by ultrasonication of soft spherical structures in the presence of plasmid DNA. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.