General method for selective labelling of double‐chain cysteine‐rich peptides with a lanthanide chelate via solid‐phase synthesis | Zendy

Shabanpoor Fazel | Zendy; Separovic Frances | Zendy; Wade John D. | Zendy

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General method for selective labelling of double‐chain cysteine‐rich peptides with a lanthanide chelate via solid‐phase synthesis

Author(s) -

Shabanpoor Fazel,

Separovic Frances,

Wade John D.

Publication year - 2011

Publication title -

journal of peptide science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 66

eISSN - 1099-1387

pISSN - 1075-2617

DOI - 10.1002/psc.1307

Subject(s) - cysteine , lanthanide , chelation , chemistry , labelling , solid phase synthesis , phase (matter) , combinatorial chemistry , biochemistry , peptide , organic chemistry , enzyme , ion

The use of lanthanides in preference to radioisotopes as probes for various biological assays has gained enormous popularity. The introduction of lanthanide chelates to peptides/proteins can be carried out either in solution using a commercially available labelling kit or by solid‐phase peptide synthesis using an appropriate lanthanide chelate. Herein, a detailed protocol for the latter is provided for the labelling of peptides or small proteins with diethylenetriamine‐ N , N , N ″, N ″‐tetra‐tert‐butyl acetate‐ N ′‐acetic acid (DTPA) chelate or other similar chelates on a solid support using a chimeric insulin‐like peptide composed of human insulin‐like peptide 5 (INSL5) A‐chain and relaxin‐3 B‐chain as a model peptide. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd.

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