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Probing protein function by chemical modification
Author(s) -
Wu YaoWen,
Goody Roger S.
Publication year - 2010
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.1287
Subject(s) - protein function , computational biology , function (biology) , posttranslational modification , chemical modification , chemical biology , chemistry , computer science , combinatorial chemistry , biochemistry , biology , microbiology and biotechnology , enzyme , gene
Labeling proteins with synthetic probes, such as fluorophores, affinity tags, and other functional labels is enormously useful for characterizing protein function in vitro , in live cells, or in whole organisms. Recent advancements of chemical methods have substantially expanded the tools that are applicable to modify proteins. In this review, we discuss some important chemical methods for site‐specific protein modification and highlight the application of established techniques to tackle biological questions. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.