Premium
Monitoring the allyl ester deprotection by HR MAS NMR in BAL‐solid phase peptide synthesis
Author(s) -
Duchène T.,
Mihai C.,
Willem R.,
Tourwé D.
Publication year - 2010
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.1278
Subject(s) - amide , linker , chemistry , magic angle spinning , peptide , solid phase synthesis , peptide synthesis , cyclic peptide , combinatorial chemistry , nuclear magnetic resonance spectroscopy , stereochemistry , organic chemistry , biochemistry , computer science , operating system
The backbone amide linker strategy, in which the growing peptide chain is anchored to a solid support via a backbone amide nitrogen, has proven to be successful for the synthesis of cyclic peptides. Optimisation of the reaction conditions for the synthesis of c(Gly‐Trp‐βAla‐Phe) could be accomplished by the help of high resolution magic angle spinning (HR MAS) NMR and the results are presented here. Signal vanishing of HR MAS NMR resonances were encountered and proven to be originated from interchain aggregations of peptide chains. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.