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Cell selectivity‐membrane phospholipids relationship of the antimicrobial effects shown by pleurocidin enantiomeric peptides
Author(s) -
Lee Juneyoung,
Park Cana,
Park SeongCheol,
Woo EunRhan,
Park Yoonkyung,
Hahm KyungSoo,
Lee Dong Gun
Publication year - 2009
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.1157
Subject(s) - enantiomer , antimicrobial , chemistry , liposome , membrane , stereochemistry , ergosterol , biochemistry , organic chemistry
Previously, we investigated the antimicrobial properties of pleurocidin (Ple) enantiomers. Our studies showed that the L ‐enantiomer exhibited about a 2–16 fold more potent activity against bacterial strains as compared to that of the D –enantiomer. However, fungal strains were about two–fold more susceptible to the D –enantiomer than to the L ‐enantiomer. In this study, confocal laser scanning microscopy indicates that the Ple enantiomers internalize into the cell surface. The present results also suggest that they could be characterized by a membrane–active mechanism. To further elucidate their selective membranolytic activities, we conducted a fluorescence analysis. A study with 1,6–diphenyl–1,3,5–hexatriene, a hydrophobic molecule, showed that the L –and the D –enantiomer exert more potent antibacterial or antifungal activity than their opposite enantiomer, respectively. Furthermore, we synthesized liposomes by using representative phospholipids consisting of bacterial or fungal membranes. Our results show that the L ‐enantiomer causes significant dye leakage from negatively charged liposomes (PG/CL; 58:42, PC/PG; 1:1, w/w) which mimic bacterial membranes such as Staphylococcus aureus . Conversely, the D –enantiomer has more potent leakage effects against fungal liposomes (PC/PE/PI/ergosterol; 5:4:1:2, w/w/w/w, PC/ergosterol; 10:1, w/w). In summary, these results suggest that the selective antimicrobial effects of the Ple enantiomers against bacterial and fungal cells may be due to the different lipid compositions of prokaryotes and eukaryotes. Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd.