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Immobilization strategies for small molecule, peptide and protein microarrays
Author(s) -
Köhn Maja
Publication year - 2009
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.1130
Subject(s) - bioorthogonal chemistry , peptide , chemistry , dna microarray , combinatorial chemistry , ligation , computational biology , biochemistry , nanotechnology , click chemistry , microbiology and biotechnology , biology , materials science , gene expression , gene
Protein, peptide and small molecule microarrays are valuable tools in biological research. In the last decade, substantial progress has been achieved to make these powerful technologies more reliable and available for researchers. This review describes chemical preparation methods for these microarrays with focus on site‐selective and bioorthogonal immobilization reactions, particularly the Staudinger ligation and the thiol‐ene reaction. In addition, the application of peptide microarrays, which were prepared by Staudinger ligation, to substrate specificity mapping is illustrated. Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd.