Resin comparison and fast automated stepwise conventional synthesis of human SDF‐1α | Zendy

Patel Hirendra | Zendy; Chantell Christina A. | Zendy; Fuentes German | Zendy; Menakuru Mahendra | Zendy; Park Jae H. | Zendy

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Resin comparison and fast automated stepwise conventional synthesis of human SDF‐1α

Author(s) -

Patel Hirendra,

Chantell Christina A.,

Fuentes German,

Menakuru Mahendra,

Park Jae H.

Publication year - 2008

Publication title -

journal of peptide science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 66

eISSN - 1099-1387

pISSN - 1075-2617

DOI - 10.1002/psc.1056

Subject(s) - peptide , chemistry , reagent , mass spectrometry , chromatography , high performance liquid chromatography , amino acid , peptide synthesis , combinatorial chemistry , organic chemistry , biochemistry

Human SDF‐1α contains 68 amino acids and is a member of the chemokine family of peptides. This long peptide was synthesized stepwise using classical conditions in 101 h. The reaction times were then reduced to deprotection times of 2 × 2 min and coupling times of 2 × 2.5 min, resulting in a total synthesis time of 22 h. The effect of different resins, resin substitutions and deprotection reagents on the crude peptide purities was compared. A small portion of crude peptide was purified using an RP‐HPLC column and the mass of the final product was confirmed with MALDI‐TOF mass spectrometry. Copyright © 2008 European Peptide Society and John Wiley & Sons, Ltd.

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