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Micelles by self‐assembling peptide‐conjugate amphiphile: synthesis and structural characterization
Author(s) -
Accardo Antonella,
Tesauro Diego,
Del Pozzo Luigi,
Mangiapia Gaetano,
Paduano Luigi,
Morelli Giancarlo
Publication year - 2008
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.1024
Subject(s) - conjugate , amphiphile , micelle , peptide , chemistry , characterization (materials science) , combinatorial chemistry , nanotechnology , biophysics , biochemistry , materials science , copolymer , organic chemistry , biology , aqueous solution , polymer , mathematical analysis , mathematics
Abstract The chemical synthesis by solid‐phase methods of a novel amphiphilic peptide, peptide‐conjugate amphiphile (PCA), containing in the same molecule three different functions: (i) the N , N ‐bis[2‐[bis(carboxy‐ethyl)amino]ethyl]‐L‐glutamic acid (DTPAGlu) chelating agent, (ii) the CCK8 bioactive peptide, and (iii) a hydrophobic moiety containing four alkyl chains with 18 carbon atoms each, is reported. In water solution at pH 7.4, PCA self‐assembles in very stable micelles at very low concentration [critical micellar concentration (cmc) values of 5 × 10 −7 mol kg −1 ] as confirmed by fluorescence spectroscopy. The structural characterization, obtained with small‐angle neutron scattering (SANS) measurements, indicates that the aggregates are substantially represented by ellipsoidal micelles with an aggregation number of 39 ± 2 and the two micellar axes of about 52 and 26 Å. Copyright © 2008 European Peptide Society and John Wiley & Sons, Ltd.