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Effects of ligands or substrate of insulin‐regulated aminopeptidase (IRAP) on trophoblast invasion
Author(s) -
Cohen Marie,
Wuillemin Christine,
Chai Siew Yeen,
Bischof Paul
Publication year - 2008
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.1018
Subject(s) - trophoblast , placentation , aminopeptidase , cell growth , peptide , biology , microbiology and biotechnology , cell , cancer research , biochemistry , medicine , chemistry , placenta , fetus , pregnancy , leucine , amino acid , genetics
Abstract Insulin‐regulated aminopeptidase (IRAP) activity increases during placentation and in the invasive tumor cell of trophoblast suggesting a role for this peptidase in the invasiveness of normal and malignant trophoblast. To investigate this hypothesis, we studied the effects of substrate (OT) and inhibitors (angiotensin peptides and LVV‐H7) of IRAP on the first trimester trophoblast proliferation and invasion. Addition of these peptides in the culture medium of trophoblastic cells significantly decreased metalloproteinase‐9 activity and cellular invasiveness while no effect was observed on cell proliferation. The peptide IRAP inhibitors could exert their effect on cytotrophoblastic cell invasiveness by inhibition of its enzymatic activity, and thus increasing half life of the known placental peptide substrate of IRAP, OT. Copyright © 2008 European Peptide Society and John Wiley & Sons, Ltd.