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A simple method for monitoring the cysteine content in synthetic peptides
Author(s) -
Horváti Kata,
Bõsze Szilvia,
Hudecz Ferenc,
MedzihradszkySchweiger Hedvig
Publication year - 2008
Publication title -
journal of peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 66
eISSN - 1099-1387
pISSN - 1075-2617
DOI - 10.1002/psc.1012
Subject(s) - simple (philosophy) , cysteine , chemistry , combinatorial chemistry , computational biology , biochemistry , computer science , biology , enzyme , philosophy , epistemology
A new method for the determination of the cysteine content of synthetic peptides is described. For this purpose the classical amino acid analysis is not suitable, as cysteine may undergo oxidative dimerisation to cystine during the hydrolysis or evaporation. The intact peptide was reacted with N ‐ethylmaleimide (NEM) yielding a stable S ‐( N ‐ethylsuccinimido)‐cysteine derivative, which could be separated by RP‐HPLC and characterised by mass spectrometry. For the quantitative determination less than 0.1 µ M peptide was sufficient. Copyright © 2008 European Peptide Society and John Wiley & Sons, Ltd.