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The effect of piperonyl butoxide on pyrethroid‐resistance‐associated esterases in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)
Author(s) -
Young Susan J,
Gunning Robin V,
Moores Graham D
Publication year - 2005
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.996
Subject(s) - piperonyl butoxide , helicoverpa armigera , noctuidae , pyrethroid , esterase , biology , toxicology , lepidoptera genitalia , biochemistry , botany , enzyme , pesticide , agronomy
Pyrethroid resistance in field populations of Australian Helicoverpa armigera (Hübner) is primarily a consequence of the overproduction of esterase isoenzymes which metabolise and sequester pyrethroid insecticides. Biochemical studies have shown that pyrethroid‐resistance‐associated esterases in H armigera are inhibited by the insecticide synergist piperonyl butoxide (PBO). Esterase inhibition by PBO did not occur immediately after dosing, but exhibited maximum inhibition 3–4 h after dosage. Esterase activity subsequently recovered until full activity was restored by 24 h. Topical bioassays using a pre‐treatment of PBO showed that maximum H armigera mortality was achieved with pre‐treatment times corresponding to maximum esterase inhibition. These results demonstrated that, with correct temporal application, PBO can restore pyrethroid efficacy against H armigera . It would also be expected that restoration of efficacy with other conventional insecticides, currently compromised by esterase‐based resistance mechanisms, would occur. Copyright © 2005 Society of Chemical Industry