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Potential determination of aminated pesticides and metabolites by cyclodextrin capillary electrophoresis–laser‐induced fluorescence using FITC as labelling
Author(s) -
NavarreteCasas Miguel,
SeguraCarretero Antonio,
CrucesBlanco Carmen,
FernándezGutiérrez Alberto
Publication year - 2005
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.961
Subject(s) - derivatization , capillary electrophoresis , chromatography , chemistry , detection limit , cyclodextrin , laser induced fluorescence , fluorescein isothiocyanate , fluorescence , fluorescein , fluorescence spectroscopy , pesticide , isothiocyanate , mass spectrometry , organic chemistry , physics , quantum mechanics , agronomy , biology
We describe a detailed study on the possibility of analyzing aminated pesticides and metabolites using pre‐column derivatization with fluorescein isothiocyanate (FITC) and their subsequent separation and detection by cyclodextrin capillary electrophoresis–laser‐induced fluorescence (CE‐LIF) detection. Different variables affecting the derivatization reaction (pH, FITC concentration, reaction time and temperature) and those related with the separation itself (buffer concentration, addition of various organic modifiers, pH, applied voltage and injection time) were studied. The limit of detection obtained was between 0.45 and 3.48 µg litre −1 showing a relative standard deviation between 0.26 and 2.08% at a concentration level of 50 µg litre −1 . Copyright © 2004 Society of Chemical Industry