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Use of the RFLP‐PCR diagnostic test for characterizing MACE and kdr insecticide resistance in the peach potato aphid Myzus persicae
Author(s) -
Cassanelli Stefano,
Cerchiari Barbara,
Giannini Sara,
Bizzaro Davide,
Mazzoni Emanuele,
Manicardi Gian Carlo
Publication year - 2005
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.954
Subject(s) - myzus persicae , aphid , aphididae , biology , insecticide resistance , horticulture , mace , restriction fragment length polymorphism , botany , homoptera , pest analysis , polymerase chain reaction , toxicology , genetics , gene , medicine , psychiatry , myocardial infarction , conventional pci
The peach‐potato aphid Myzus persicae (Sulzer) has developed a number of insecticide resistance mechanisms owing to the high selective pressure produced by world‐wide insecticide treatments. Knowledge of the geographical distribution and the temporal evolution of these resistant phenotypes helps to develop suitable pest‐management programs. Current understanding of the major mechanisms of resistance at the molecular level makes it possible to diagnose the presence of modified acetylcholinesterase (MACE) or knockdown resistance ( kdr ). This paper describes a rapid method for the identification of both resistance mechanisms in a single molecular assay by using restriction fragment length polymorphism of PCR products (RFLP‐PCR) in individual as well as pooled aphids. Copyright © 2004 Society of Chemical Industry