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Diagnosis of dehydratase inhibitors in melanin biosynthesis inhibitor (MBI‐D) resistance by primer‐introduced restriction enzyme analysis in scytalone dehydratase gene of Magnaporthe grisea
Author(s) -
Kaku Koichiro,
Takagaki Makiichi,
Shimizu Tsutomu,
Nagayama Kozo
Publication year - 2003
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.742
Subject(s) - magnaporthe grisea , dehydratase , primer (cosmetics) , enzyme , biology , fungus , restriction enzyme , gene , biochemistry , chemistry , botany , oryza sativa , organic chemistry
We have established a simple diagnosis method for rice blast fungus resistant to MBI‐D. This involves the preparation of PCR templates directly from the lesions in combination with primer‐introduced restriction enzyme analysis PCR (PIRA‐PCR). Copyright © 2003 Society of Chemical Industry