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Correlations of the electrophysiological activity of neonicotinoids with their binding and insecticidal activities
Author(s) -
Nishiwaki Hisashi,
Nakagawa Yoshiaki,
Kuwamura Morihiko,
Sato Kazuyuki,
Akamatsu Miki,
Matsuda Kazuhiko,
Komai Koichiro,
Miyagawa Hisashi
Publication year - 2003
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.729
Subject(s) - xenopus , imidacloprid , agonist , acetylcholine receptor , neonicotinoid , nicotinic agonist , nicotinic acetylcholine receptor , acetylcholine , pharmacology , in vivo , chemistry , electrophysiology , biology , receptor , biochemistry , neuroscience , pesticide , genetics , ecology , gene
The electrophysiological actions of various neonicotinoids, including substituted benzyl derivatives, against recombinant Drosophila SAD/chicken β2 hybrid nicotinic acetylcholine receptor (nAChR) were measured to analyze the relationships between the in vivo (insecticidal) and in vitro (binding and agonist) activities. Most of the neonicotinoids tested were capable of inducing inward currents by activating the hybrid nAChRs expressed in Xenopus laevis oocytes, whereas some compounds had no agonist activity and only blocked the acetylcholine‐induced currents. Variations in the agonist activity were well correlated with those in the binding potency evaluated using [ 3 H]imidacloprid as well as insecticidal activities. Copyright © 2003 Society of Chemical Industry