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Ectopic expression of a rice triketone dioxygenase gene confers mesotrione tolerance in soybean
Author(s) -
Dai Shunhong,
Georgelis Nikolaos,
Bedair Mohamed,
Hong YunJeong,
Qi Qungang,
Larue Clayton T,
Sitoula Bikram,
Huang Wei,
Krebel Brian,
Shepard Michael,
Su Wen,
Kretzmer Keith,
Dong Jiaxin,
Slewinski Thomas,
Berger Sarah,
Ellis Christine,
Jerga Agoston,
Varagona Marguerite
Publication year - 2022
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.6904
Subject(s) - mesotrione , biology , genetically modified crops , ectopic expression , microbiology and biotechnology , transgene , gene , agronomy , biochemistry , pesticide , atrazine
Abstract BACKGROUND Herbicide‐resistant weeds pose a challenge to agriculture and food production. New herbicide tolerance traits in crops will provide farmers with more options to effectively manage weeds. Mesotrione, a selective pre‐ and post‐emergent triketone herbicide used in corn production, controls broadleaf and some annual grass weeds via hydroxyphenylpyruvate dioxygenase (HPPD) inhibition. Recently, the rice HIS1 gene, responsible for native tolerance to the selective triketone herbicide benzobicyclon, was identified. Expression of HIS1 also confers a modest level of mesotrione resistance in rice. Here we report the use of the HIS1 gene to develop a mesotrione tolerance trait in soybean. RESULTS Conventional soybean is highly sensitive to mesotrione. Ectopic expression of a codon‐optimized version of the rice HIS1 gene ( TDO ) in soybean confers a commercial level of mesotrione tolerance. In TDO transgenic soybean plants, mesotrione is rapidly and locally oxidized into noninhibitory metabolites in leaf tissues directly exposed to the herbicide. These metabolites are further converted into compounds similar to known classes of plant secondary metabolites. This rapid metabolism prevents movement of mesotrione from treated leaves into vulnerable emerging leaves. Minimizing the accumulation of the herbicide in vulnerable emerging leaves protects the function of HPPD and carotenoid biosynthesis more generally while providing tolerance to mesotrione. CONCLUSIONS Mesotrione has a favorable environmental and toxicological profile. The TDO ‐mediated soybean mesotrione tolerance trait described here provides farmers with a new option to effectively manage difficult‐to‐control weeds using familiar herbicide chemistry. This trait can also be adapted to other mesotrione‐sensitive crops (e.g. cotton) for effective weed management. © 2022 Bayer Crop Science. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.