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Genetic architecture underlying HPPD ‐inhibitor resistance in a Nebraska Amaranthus tuberculatus population
Author(s) -
Murphy Brent P,
Beffa Roland,
Tranel Patrick J
Publication year - 2021
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.6560
Subject(s) - biology , quantitative trait locus , weed , population , genetic architecture , trait , resistance (ecology) , genetics , botany , agronomy , gene , demography , sociology , computer science , programming language
BACKGROUND Amaranthus tuberculatus is a problematic weed species in Midwest USA agricultural systems. Inhibitors of 4‐hydroxyphenylpyruvate dioxygenase (HPPD) are an important chemistry for weed management in numerous cropping systems. Here, we characterize the genetic architecture underlying the HPPD‐inhibitor resistance trait in an A. tuberculatus population (NEB). RESULTS Dose–response studies of an F 1 generation identified HPPD‐inhibitor resistance as a dominant trait with a resistance factor of 15.0–21.1 based on dose required for 50% growth reduction. Segregation analysis in a pseudo‐F 2 generation determined the trait is moderately heritable ( H 2 = 0.556) and complex. Bulk segregant analysis and validation with molecular markers identified two quantitative trait loci (QTL), one on each of Scaffold 4 and 12. CONCLUSIONS Resistance to HPPD inhibitors is a complex, largely dominant trait within the NEB population. Two large‐effect QTL were identified controlling HPPD‐inhibitor resistance in A. tuberculatus . This is the first QTL mapping study to characterize herbicide resistance in a weedy species.