Premium
Characterization of Hungarian isolates of zucchini yellow mosaic virus (ZYMV, potyvirus) transmitted by seeds of Cucurbita pepo var Styriaca
Author(s) -
Tóbiás István,
Palkovics László
Publication year - 2003
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.645
Subject(s) - cucurbita pepo , zucchini yellow mosaic virus , biology , phylogenetic tree , potyvirus , botany , squash , cucurbita , papaya ringspot virus , plant virus , cucurbita moschata , virus , virology , genetics , gene , medicine , alternative medicine , pathology
Zucchini yellow mosaic virus (ZYMV) has emerged as an important pathogen of cucurbits within the last few years in Hungary. The Hungarian isolates show a high biological variability, have specific nucleotide and amino acid sequences in the N ‐terminal region of coat protein and form a distinct branch in the phylogenetic tree. The virus is spread very efficiently in the field by several aphid species in a non‐persistent manner. It can be transmitted by seed in holl‐less seeded oil pumpkin ( Cucurbita pepo (L) var Styriaca ), although at a very low rate. Three isolates from seed transmission assay experiments were chosen and their nucleotide sequences of coat proteins have been compared with the available CP sequences of ZYMV. According to the sequence analysis, the Hungarian isolates belong to the Central European branch in the phylogenetic tree and, together with the ZYMV isolates from Austria and Slovenia, share specific amino acids at positions 16, 17, 27 and 37 which are characteristic only to these isolates. The phylogenetic tree suggests the common origin of distantly distributed isolates which can be attributed to widespread seed transmission. © 2003 Society of Chemical Industry