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Identification of ABCG transporter genes associated with chlorantraniliprole resistance in Plutella xylostella (L.)
Author(s) -
Shan Jinqiong,
Sun Xi,
Li Ran,
Zhu Bin,
Liang Pei,
Gao Xiwu
Publication year - 2021
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.6402
Subject(s) - plutella , atp binding cassette transporter , diamondback moth , biology , gene knockdown , abcg2 , population , gene , pharmacology , transporter , genetics , botany , larva , medicine , environmental health
Abstract BACKGROUND Plutella xylostella (L.) is a serious worldwide pest that feeds on cruciferous plants and has evolved resistance to different classes of insecticides used for its control, including chlorantraniliprole. ATP‐binding cassette (ABC) transporters, constituting the largest transport family in organisms, are involved in phase III of the detoxification process and may play important roles in insecticide resistance. RESULTS A total of 15 ABC transporter transcripts from subfamily G were identified in P. xylostella based on the latest DBM genome. Synergism studies showed that treatment with verapamil, a potent inhibitor of ABC transporters, significantly increased the toxicity of chlorantraniliprole against larvae of two chlorantraniliprole‐resistant P. xylostella populations (NIL and BL). ABCG2 , ABCG5 , ABCG6 , ABCG9 , ABCG11 , ABCG14 and ABCG15 were significantly overexpressed in NIL and BL compared with the susceptible population (SS), and ABCG1 , ABCG6 , ABCG8 , ABCG9 , ABCG14 and ABCG15 were significantly upregulated after treatment with the LC 50 of chlorantraniliprole in SS. Subsequently, ABCG6 , ABCG9 and ABCG14 , which were overexpressed in both NIL and BL and could be induced in SS, were chosen for functional study. RNAi‐mediated knockdown of each of the three ABCG s significantly increased the sensitivity of larvae to chlorantraniliprole. These results confirmed that overexpression of ABCG6 , ABCG9 and ABCG14 may contribute to chlorantraniliprole resistance in P. xylostella . CONCLUSION Overexpression of some genes in the ABCG subfamily is involved in P. xylostella resistance to chlorantraniliprole. These results may help to establish a foundation for further studies investigating the role played by ABC transporters in chlorantraniliprole resistance in P. xylostella or other insect pests. © 2021 Society of Chemical Industry.