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Development of ‘multiresistance rice’ by an assembly of herbicide, insect and disease resistance genes with a transgene stacking system
Author(s) -
Li Chuanxu,
Zhang Jianguo,
Ren Zhiyong,
Xie Rong,
Yin Changxi,
Ma Weihua,
Zhou Fei,
Chen Hao,
Lin Yongjun
Publication year - 2021
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.6178
Subject(s) - brown planthopper , biology , cultivar , genetically modified rice , oryza sativa , microbiology and biotechnology , agronomy , pest analysis , blight , resistance (ecology) , genetically modified crops , transgene , gene , botany , genetics
Background Weeds, diseases and pests pose serious threats to rice production and cause significant economic losses. Cultivation of rice varieties with resistance to herbicides, diseases and pests is believed to be the most economical and environmentally friendly method to deal with these problems. Results In this study, a highly efficient transgene stacking system was used to assembly the synthetic glyphosate‐tolerance gene ( I. variabilis‐EPSPS* ), lepidopteran pest resistance gene ( Cry1C* ), brown planthopper resistance genes ( Bph14* and OsLecRK1* ), bacterial blight resistance gene ( Xa23* ) and rice blast resistance gene ( Pi9* ) onto a transformable artificial chromosome vector. The construct was transferred into ZH11 (a widely used japonica rice cultivar Zhonghua 11) via Agrobacterium ‐mediated transformation and ‘multiresistance rice’ (MRR) with desirable agronomic traits was obtained. The results showed that MRR had significantly improved resistance to glyphosate, borers, brown planthopper, bacterial blight and rice blast relative to the recipient cultivar ZH11. Besides, under the natural occurrence of pests and diseases in the field, the yield of MRR was significantly higher than that of ZH11. Conclusion A multigene transformation strategy was employed to successfully develop rice lines with multiresistance to glyphosate, borers, brown planthopper, bacterial blight and rice blast, and the obtained MRR is expected to have great application potential. © 2020 Society of Chemical Industry