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Current scenario of RNAi ‐based hemipteran control
Author(s) -
Jain Ritesh G,
Robinson Karl E,
Asgari Sassan,
Mitter Neena
Publication year - 2021
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.6153
Subject(s) - rna interference , rna silencing , biology , effector , gene silencing , function (biology) , computational biology , gene , genetics , microbiology and biotechnology , rna
RNA interference (RNAi) is an homology‐dependent gene silencing mechanism that is a feasible and sustainable avenue for the management of hemipteran pests. Commercial implementation of RNAi‐based control strategies is impeded by limited knowledge about the mechanism of double‐stranded RNA (dsRNA) uptake, the function of core RNAi genes and systemic RNAi mechanisms in hemipteran insects. This review briefly summarizes recent progress in RNAi‐based studies aimed to reduce insect populations, viral transmission and insecticide resistance focusing on hemipteran pests. This review explores RNAi‐mediated management of hemipteran insects and offers potential solutions, including in silico approaches coupled with laboratory‐based toxicity assays to circumvent potential off‐target effects against beneficial organisms. We further explore ways to mitigate degradation of dsRNA in the environment and the insect such as stacking and formulation of dsRNA effectors. Finally, we conclude by considering nontransformative RNAi approaches, concatomerization of RNAi sequences and pyramiding RNAi with active constituents to reduce dsRNA production and application cost, and to improve broad‐spectrum hemipteran pest control. © 2020 Society of Chemical Industry