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Bacillus amyloliquefaciens strain QST713 may contribute to the management of SDHI resistance in Botrytis cinerea
Author(s) -
Samaras Anastasios,
Hadjipetrou Chloe,
Karaoglanidis George
Publication year - 2021
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.6145
Subject(s) - botrytis cinerea , bacillus amyloliquefaciens , mutant , fungicide , biology , horticulture , microbiology and biotechnology , food science , biochemistry , fermentation , gene
BACKGROUND Resistance of Botrytis cinerea to SDHI fungicides is widely distributed throughout the world and is associated with mutations in sdh B, differentially affecting mutant sensitivity to several succinate dehydrogenase inhibitors (SDHI) and the fitness of the strains. This study was initiated to test the hypothesis that Bacillus amyloliquefaciens QST713 ( Ba QST713) can be utilized in Integrated Pest Management (IPM) programs aiming to control grey mould and eliminate sdh B mutants (H272R/Y, N230I and P225F/H/L). RESULTS Protective and curative applications of Ba QST713 on artificially inoculated bean plants resulted in a significant reduction of disease incidence and severity. Competition experiments between sdh B mutants and wild‐type isolates conducted either in the absence of any treatment or in the presence of Ba QST713 or fluopyram showed a dominance of sensitive strains over the mutated strains on untreated and Ba QST713‐treated plants. Additionally, the efficacy of Ba QST713 in controlling grey mould and its effects on the selection of sdh B mutants was assessed in a greenhouse experiment. The applications of Ba QST713 in alternation schemes with fluopyram provided high control efficacy and reduced SDHI resistance frequency. CONCLUSIONS The results of the study showed that Ba QST713 can contribute both to moderate/high levels of grey mould suppression and to a reduction in SDHI resistance frequency. Thus, Ba QST713 can be an efficient tool for SDHI resistance management of B. cinerea in the field. © 2020 Society of Chemical Industry