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Isolation of a Pseudomonas fluorescens metabolite/exotoxin active against both larvae and pupae of vector mosquitoes
Author(s) -
Prabakaran G,
Paily K P,
Padmanabhan V,
Hoti S L,
Balaraman K
Publication year - 2003
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.610
Subject(s) - aedes aegypti , anopheles stephensi , culex quinquefasciatus , biology , larva , pupa , pseudomonas fluorescens , instar , metabolite , biological pest control , microbiology and biotechnology , veterinary medicine , botany , bacteria , biochemistry , medicine , genetics
A formulation was developed from the metabolite(s) of a novel Pseudomonas fluorescens Migula strain (VCRC B426) and tested against 4th‐instar larvae and pupae of three species of vector mosquitoes, Anopheles stephensi Liston, Culex quinquefasciatus Say and Aedes aegypti (L). The larvae and pupae of An stephensi were the most susceptible to the formulation, followed by those of C quinquefasciatus and Ae aegypti , in that order, and the dosage requirement for pupal mortality was less than that required for larval mortality. The LC 50 dosage requirements for larvae of these mosquito species were, respectively, 70.4, 511.5 and 757.3 µg protein ml −1 , whereas for pupae they were, respectively, 2.0, 9.4 and 19.2 µg protein ml −1 . The lethal fraction was purified from the culture broth and its molecular mass, as determined by high performance liquid chromatography, was 44 kDa. This is the first report of a microbial formulation acting upon mosquito pupae, a non‐feeding stage. Its mode of action and efficacy to control mosquitoes under field conditions need to be studied further. © 2002 Society of Chemical Industry