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Origin, selection, and spread of diamide insecticide resistance allele in field populations of diamondback moth in east and southeast Asia
Author(s) -
Uesugi Ryuji,
Jouraku Akiya,
Sukonthabhirom na Pattalung Suprada,
Hinomoto Norihide,
Kuwazaki Seigo,
Kanamori Hiroyuki,
Katayose Yuichi,
Sonoda Shoji
Publication year - 2021
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.6020
Subject(s) - diamondback moth , biology , allele , plutella , genetics , overwintering , allele frequency , mutation , resistance (ecology) , pesticide resistance , insecticide resistance , gene , microbiology and biotechnology , ecology , toxicology , larva , pesticide
BACKGROUND The investigation of molecular mechanisms and evolution of resistance to insecticides is an ongoing challenge, as researchers must provide guidance to manage the resistance to achieve sustainable production in agriculture. Predicting, monitoring, and managing insecticide resistance requires information on the origins, selection, and spread of resistance genes. The resistance of Plutella xylostella (L.) against diamide insecticides is becoming an increasingly severe problem in east and southeast Asia. In this study, the evolution of resistance was investigated using a resistance allele [ryanodine receptor ( RyR ); G4946E mutation] and its flanking regions, as well as mitochondrial cytochrome c oxidase subunit I ( mtCOI ). RESULTS The sequences of the flanking region of the G4946E and mtCO I suggested that the G4946E mutation has a key role in resistance. Furthermore, the G4946E mutation has multiple origins, and congenic resistant mutations have spread across east and southeast Asia, despite substantial geographical barriers. In addition, the susceptibility of field populations partially recovered during winter, based on the observed decrease in the G4946E (resistant allele) frequency. Finally, the resistance level indexed by the frequency of the E4946 allele was significantly lower in non‐overwintering regions than in overwintering regions. CONCLUSION The information of the present study is useful to monitor resistance using molecular markers and to develop strategies to delay the evolution of diamide resistance.