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Production of patchouli mild mosaic virus resistant patchouli plants by genetic engineering of coat protein precursor gene
Author(s) -
Kadotani Naoto,
Ikegami Masato
Publication year - 2002
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.581
Subject(s) - patchouli , pogostemon , biology , genetically modified crops , southern blot , gene , transformation (genetics) , agrobacterium , horticulture , botany , transgene , essential oil , genetics , traditional medicine , medicine
Patchouli ( Pogostemon cablin (Blanco) Benth), an aromatic crop which yields an essential oil, is widely cultivated in South‐east Asia. Patchouli mild mosaic virus (PaMMV) infects patchouli plants and causes decrease in leaf biomass and essential oil yield. Transgenic patchouli plants with PaMMV coat protein precursor (CP‐P) gene have been produced by Agrobacterium ‐mediated transformation. PaMMV CP‐P gene integration into the patchouli genome was confirmed by the PCR method and by Southern blot analysis. The transformants were estimated to contain one to three copy genes using Southern blot analysis. The transformant with three copy genes was tested for the resistance to PaMMV by artificially inoculating plants grown in an environmentally controlled cabinet, and this transformant was found to be highly resistant to PaMMV. The transgenic patchouli plant with PaMMV CP‐P gene should provide valuable material for protecting against PaMMV. © 2002 Society of Chemical Industry