Premium
Triazophos‐induced vertical transmission of rice stripe virus is associated with host vitellogenin in the small brown planthopper Laodelphax striatellus
Author(s) -
Xu Gang,
Jiang Yu,
Zhang Nannan,
Liu Fang,
Yang Guoqing
Publication year - 2020
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5729
Subject(s) - brown planthopper , biology , horizontal transmission , gene knockdown , vitellogenin , pest analysis , transmission (telecommunications) , host (biology) , virology , virus , horticulture , genetics , gene , electrical engineering , engineering
BACKGROUND Rice stripe virus (RSV) is one of the most serious rice diseases in East Asia. The small brown planthopper (SBPH), Laodelphax striatellus , is an economically important rice pest. SBPH transmits RSV horizontally and vertically, resulting in serious rice economic losses. Exposure to sublethal doses of insecticides can induce many physiological and cellular changes in insects. However, the mechanism underlying triazophos‐induced RSV vertical transmission remains unclear. Here, we investigated the role of vitellogenin (Vg) in triazophos‐induced RSV vertical transmission. RESULTS RSV vertical transmission rates were significantly induced by sublethal exposure to triazophos. The transcript abundance of viral RNA3 segment ( RNA3 ) and capsid protein ( CP ) in the ovaries of SBPH were also significantly increased. Triazophos induced the expression level of Vg in L. striatellus ( LsVg ) and increased the contents of ovarian protein and fat body protein. Knockdown of Vg significantly reduced the expression levels of LsVg and Vg receptor ( LsVgR ), and decreased RSV accumulations in the ovaries. Double‐stranded Vg (dsVg)‐mediated down‐regulation could be rescued by exposure to triazophos. Vertical transmission rate of the dsVg‐injected group was significantly decreased compared with the dsGFP‐injected group, and triazophos significantly rescued the RSV vertical transmission rate of the dsVg‐injected group. CONCLUSION Our results indicate that triazophos‐induced RSV vertical transmission is associated with Vg. This work will help us to further elucidate sublethal doses of insecticides‐mediated effects and develop new strategies for pest control. © 2019 Society of Chemical Industry