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Antifeedant, contact toxicity and oviposition deterrent effects of phyllostine acetate and phyllostine isolated from the endophytic fungus Diaporthe miriciae against Plutella xylostella larvae
Author(s) -
Ratnaweera Pamoda B,
M Jayasundara J M Nisansala,
Herath H H M Suseema D,
Williams David E,
Rajapaksha Sasini U,
Nishantha K M D W Prabath,
Silva E Dilip,
Andersen Raymond J
Publication year - 2020
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5673
Subject(s) - plutella , plant use of endophytic fungi in defense , diamondback moth , biology , pest analysis , pesticide , larva , toxicology , pest control , ethyl acetate , toxicity , botany , horticulture , agronomy , chemistry , organic chemistry , biochemistry
BACKGROUND Larvae of the diamond back moth (DBM), Plutella xylostella , are destructive cabbage pests causing economic losses worldwide. Continuous application of synthetic pesticides to control this pest has resulted in environmental pollution and resistant pest strains. Thus, there is a crucial need to seek natural alternatives with minimal detrimental effects. This study was designed to investigate the antifeedant activities of endophytic fungi of Cyperus iria and to determine the antifeedant, contact toxicity and oviposition deterrent activities of phyllostine acetate and phyllostine of the endophytic Diaporthe miriciae fungus. RESULTS Two cyclohexeneoxidediones, phyllostine acetate ( 1 ) and phyllostine ( 2 ), isolated from an ethyl acetate extract of D. miriciae exhibited strong antifeedant, contact toxicity, and oviposition deterrent activities against P. xylostella . Phyllostine acetate ( 1 ) and phyllostine ( 2 ) showed feeding deterrent indexes of 100% at 50 μg cm −2 in the no‐choice leaf disc assay and 50% feeding deterrence (DC 50 ) values of 9 and 4.7 μg cm −2 respectively. The median lethal concentration (LC 50 ) values of phyllostine acetate ( 1 ) and phyllostine ( 2 ) were 4.38 and 6.54 μg/larva in the contact toxicity assay. The oviposition deterrent indexes of the two compounds were 100% for phyllostine acetate ( 1 ) and 28.6% for phyllostine ( 2 ) at 50 μg cm −2 . CONCLUSION Phyllostine acetate and phyllostine show promise as compounds for the control of P. xylostella . This study encourages further investigation of endophytic fungi of the family Cyperacea, for the development of natural pest control agents in agriculture. © 2019 Society of Chemical Industry