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Microbial HemG‐type protoporphyrinogen IX oxidase enzymes for biotechnology applications in plant herbicide tolerance traits
Author(s) -
Larue Clayton T,
Ream Joel E,
Zhou Xuefeng,
Moshiri Farhard,
Howe Arlene,
Goley Michael,
Sparks Oscar C,
Voss Steven T,
Hall Erin,
Ellis Christine,
Weihe Janice,
Qi Qungang,
Ribeiro Daniela,
Wei Xiaoping,
Guo Shirley,
Evdokimov Artem G,
Varagona Marguerite J,
Roberts James K
Publication year - 2020
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5613
Subject(s) - protoporphyrinogen oxidase , enzyme , biology , biochemistry , polyphenol oxidase , peroxidase
Abstract BACKGROUND Protoporphyrinogen IX oxidase (PPO)‐inhibiting herbicides act by inhibiting a key enzyme in the heme and chlorophyll biosynthetic pathways in plants. This enzyme, the PPO enzyme, is conserved across plant species. However, some microbes are known to utilize a unique family of PPO enzymes, the HemG family. This enzyme family carries out the same enzymatic step as the plant PPO enzymes, but does not share sequence homology with the plant PPO enzymes. RESULTS Bioinformatic analysis was used to identify putative HemG PPO enzyme variants from microbial sources. A subset of these variants was cloned and characterized. HemG PPO variants were characterized for functionality and tolerance to PPO‐inhibiting herbicides. HemG PPO variants that exhibited insensitivity to PPO‐inhibiting herbicides were identified for further characterization. Expression of selected variants in maize, soybean, cotton and canola resulted in plants that displayed tolerance to applications of PPO‐inhibiting herbicides. CONCLUSION Selected microbial‐sourced HemG PPO enzyme variants present an opportunity for building new herbicide tolerance biotechnology traits. These traits provide tolerance to PPO‐inhibiting herbicides and, therefore, could provide additional tools for farmers to employ in their weed management systems. © 2019 Society of Chemical Industry