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The cytochrome P450 CYP389C16 contributes to the cross‐resistance between cyflumetofen and pyridaben in Tetranychus cinnabarinus (Boisduval)
Author(s) -
Feng Kaiyang,
Ou Shiyuan,
Zhang Ping,
Wen Xiang,
Shi Li,
Yang Yuwei,
Hu Yuan,
Zhang Yichao,
Shen Guangmao,
Xu Zhifeng,
He Lin
Publication year - 2020
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5564
Subject(s) - cross resistance , metabolite , acaricide , spider mite , cytochrome p450 , biology , tetranychus , chemistry , tetranychus urticae , biochemistry , toxicology , enzyme , botany , mite , microbiology and biotechnology
BACKGROUND Acaricide resistance is a serious problem in spider mites. Cyflumetofen is a new complex II inhibitor, whereas pyridaben acts at complex I and has been used for decades. Although cross‐resistance between cyflumetofen and pyridaben has been observed in Tetranychus cinnabarinus , the specific mechanisms at play have not yet been investigated. RESULTS Investigation into the cross‐resistance mechanisms identified five P450s, among which CYP389C16 was evaluated as the most likely candidate conferring cross‐resistance. Knockdown of CYP389C16 expression via RNA interference diminished the level of cross‐resistance in the cyflumetofen‐resistant strain. In addition, recombinant CYP389C16 (40 pmol) effectively metabolized 25.0 ± 0.7% of cyflumetofen, 39.7 ± 1.0% of pyridaben, and 69.3 ± 3.3% of AB‐1 (active de‐esterified metabolite of cyflumetofen) within 2 h. In addition, hydroxylation metabolite of AB‐1 was identified by HPLC‐MS/MS. CONCLUSIONS The study reveals that overexpressed CYP389C16 is involved in the cross‐resistance between cyflumetofen and pyridaben in T. cinnabarinus . © 2019 Society of Chemical Industry