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Neurotoxicity and mode of action of fluralaner on honeybee Apis mellifera L.
Author(s) -
Sheng ChengWang,
Huang QiuTang,
Liu GenYan,
Ren XueXiang,
Jiang Jie,
Jia ZhongQiang,
Han ZhaoJun,
Zhao ChunQing
Publication year - 2019
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5483
Subject(s) - fipronil , neurotoxicity , biology , pharmacology , abamectin , xenopus , toxicology , toxicity , in vivo , pesticide , strychnine , gaba receptor , receptor , gabaa receptor , chemistry , biochemistry , microbiology and biotechnology , ecology , gene , organic chemistry
BACKGROUND Fluralaner, a novel pesticide that targets the γ‐aminobutyric acid (GABA) receptor (GABAR) subunit of resistant to dieldrin (RDL), exhibits strong potential to be an insecticide to control agricultural insect pests. However, the risk and action of fluralaner to economic insects, e.g., honeybee Apis mellifera Linnaeus, remains unclear. RESULTS In this study, both oral and contact toxicity of fluralaner to honeybee were found to be 0.13 μg adult –1 . Abamectin, dieldrin, ethiprole, α‐endosulfan, fipronil and fluralaner strongly inhibited the GABA‐induced current in A. mellifera RDL ( Am RDL), expressed in Xenopus laevis oocytes, with median inhibitory concentration (IC 50 ) values of 7.99, 868.1, 27.10, 412.0, 11.21 and 13.59 nM, respectively. The binding free energy and electrophysiological response of Am RDL and insecticides were opposite. The correlation values between toxicity (to A. mellifera ) and binding free energy/electrophysiological inhibition (to Am RDL) were at a moderate level. CONCLUSION In conclusion, we report for the first time the notable risk of fluralaner to honeybee in vivo and compared the actions of GABAR‐targeted insecticides on the Am RDL receptor. © 2019 Society of Chemical Industry