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Transcriptome responses to the natural phytotoxin t ‐chalcone in Arabidopsis thaliana L.
Author(s) -
DíazTielas Carla,
Graña Elisa,
SánchezMoreiras Adela M,
Reigosa Manuel J,
Vaughn Justin N,
Pan Zhiqiang,
BajsaHirschel Joanna,
Duke Mary V,
Duke Stephen O
Publication year - 2019
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5405
Subject(s) - phytotoxin , transcriptome , biology , chalcone synthase , arabidopsis thaliana , biochemistry , chalcone , lemna , arabidopsis , toxin , gene , gene expression , botany , chemistry , stereochemistry , mutant
BACKGROUND New modes of action are needed for herbicides. The flavonoid synthesis intermediate t ‐chalcone causes apoptosis‐like symptoms in roots and bleaching of shoots of Arabidospsis, suggesting a unique mode of action as a phytotoxin. RESULTS Using RNA‐Seq, transcriptome changes were monitored in Arabidopsis seedlings during the first 24 h of exposure (at 1, 3, 6, 12 and 24 h) to 21 μ m t ‐chalcone (I 50 dose), examining effects on roots and shoots separately. Expression of 892 and 1000 genes was affected in roots and shoots, respectively. According to biological classification, many of the affected genes were transcription factors and genes associated with oxidative stress, heat shock proteins, xenobiotic detoxification, ABA and auxin biosynthesis, and primary metabolic processess. These are secondary effects found with most phytotoxins. Potent phytotoxins usually act by inhibiting enzymes of primary metabolism. KEGG pathway analysis of transcriptome results from the first 3 h of t ‐chalcone exposure indicated several potential primary metabolism target sites for t ‐chalcone. Of these, p ‐hydroxyphenylpyruvate dioxygenase (HPPD) and tyrosine amino transferase were consistent with the bleaching effect of the phytotoxin. Supplementation studies with Lemna paucicostata and Arabidiopsis supported HPPD as the target, although in vitro enzyme inhibition was not found. CONCLUSIONS t ‐Chalcone is possibly a protoxin that is converted to a HPPD inhibitor in vivo . © 2019 Society of Chemical Industry