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Selection for imidacloprid resistance and mode of inheritance in the brown planthopper, Nilaparvata lugens
Author(s) -
SanadaMorimura Sachiyo,
Fujii Tomohisa,
Chien Ho Van,
Cuong Le Quoc,
Estoy Gerardo F,
Matsumura Masaya
Publication year - 2019
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5364
Subject(s) - imidacloprid , brown planthopper , biology , piperonyl butoxide , pest analysis , delphacidae , genetics , mendelian inheritance , epistasis , strain (injury) , dominance (genetics) , thiamethoxam , neonicotinoid , trait , toxicology , gene , botany , agronomy , pesticide , homoptera , anatomy , computer science , programming language
BACKGROUND Strong resistance to imidacloprid in Nilaparvata lugens (Stål) has developed in Southeast and East Asia. Although the mode of inheritance for resistance is very useful information for pest control, this information is unknown in N. lugens . Here, we established two resistant strains that were selected from field populations in Vietnam and the Philippines, and conducted crossing experiments to determine the inheritance pattern. RESULTS The resistance ratio of 50% lethal dose (LD 50 ) values for the two resistance‐selected strains, i.e., resistant strains originating from Vietnam (VT‐Res) and the Philippines (PH‐Res), to their control strains were ∼ 8‐ and 157‐fold, respectively. Reciprocal cross experiments between VT‐Res and the susceptible strain (S‐strain), and between PH‐Res and the S‐strain showed that the degree of dominance was 0.81 and 0.82, and 0.95 and 0.96, respectively. Analysis of the F 2 populations and backcrosses to the parental strains indicated that resistance is a major‐gene trait following Mendelian inheritance. The strength of the resistance was suppressed by pre‐treatment with piperonyl butoxide, an inhibitor of cytochrome P450‐monooxygenases. CONCLUSION Our results suggest that imidacloprid resistance in N. lugens is autosomal and an almost completely dominant major‐gene trait that is likely manifested by high expression levels of a detoxification enzyme. © 2019 Society of Chemical Industry