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Production of anti‐virus, viroid plants by genetic manipulations
Author(s) -
Ishida Isao,
Tukahara Masayoshi,
Yoshioka Masaharu,
Ogawa Toshiya,
Kakitani Mokoto,
Toguri Toshihiro
Publication year - 2002
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.536
Subject(s) - viroid , biology , cucumber mosaic virus , potato spindle tuber viroid , tobacco mosaic virus , cucumovirus , plant virus , transformation (genetics) , genetically modified crops , virology , ribonuclease , agrobacterium , potato virus y , rna , rnase p , virus , transgene , gene , genetics
Many pathogenic plant viruses are RNA viruses, which initiate production of double‐stranded RNA intermediates when they replicate in host plant cells. Introduction of double‐stranded RNA‐specific ribonucleases such as the Schizosaccharomyces pombe derived pac I protein and animal cell derived interferon‐induced 2′,5′‐oligoadenylate synthetase (2‐5Aase)/ribonuclease L (RNase L) system into various plants may make plants resistant to various pathogenic viruses and viroids. We have demonstrated that pac I and 2‐5Aase/RNase L transgenic tobacco plants are resistant to various viruses including tobacco mosaic virus, cucumber mosaic virus and potato virus Y. In addition, pac I transgenic potato plants are resistant to potato spindle tuber viroid. Using Agrobacterium ‐mediated transformation, we have established a transformation system for chrysanthemum plants and have recently developed pac I transgenic chrysanthemum ( Dendranthema grandiflora cv Reagan) resistant to chrysanthemum stunt viroid and have grown them in isolated fields for an evaluation of their effects. © 2002 Society of Chemical Industry