Premium
Induced novel psbA mutation (Ala 251 to Thr) in higher plants confers resistance to PSII inhibitor metribuzin in Lens culinaris
Author(s) -
McMurray Larn S,
Preston Christopher,
Vandenberg Albert,
Mao Dili,
Bett Kirstin E,
Paull Jeffrey G
Publication year - 2019
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5328
Subject(s) - metribuzin , biology , mutant , photosystem ii , ethyl methanesulfonate , atrazine , mutation , genetics , botany , gene , weed control , photosynthesis , pesticide , agronomy
BACKGROUND Weed competition is a major limitation to worldwide lentil ( Lens culinaris Medik.) production in part due to limited effective safe herbicide options. Metribuzin is a photosystem II inhibiting herbicide that provides broad spectrum weed control, however it causes excessive injury in lentil. Dose response analysis of photosystem II inhibiting herbicides and DNA sequencing of the psbA chloroplast gene occurred to quantify the spectrum and mechanism of herbicide resistance in two ethyl‐methanesulfonate (EMS) induced mutant lentils. RESULTS Compared to susceptible parent PBA Flash, the level of metribuzin resistance was 33‐fold for mutant M043 and 10‐fold for M009. No improvement in resistance occurred in either mutant to bromoxynil, diuron, bromacil and atrazine herbicides. Nucleotide sequencing of the psbA gene of both mutants identified a substitution at position 751 compared to PBA Flash. The resulting deduced amino acid sequence indicated an Ala 251 Thr substitution as being most likely responsible for the high level of metribuzin resistance. CONCLUSIONS The Ala 251 Thr substitution discovered in this study is unique in mutagenized higher plants and the first report of an induced psbA target site mutation in higher plants. This target site metribuzin resistance is likely to have a significant impact on lentil production in Australia and worldwide. © 2019 Society of Chemical Industry