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Evaluation of Rice stripe virus transmission efficiency by quantification of viral load in the saliva of insect vector
Author(s) -
Li Jing,
Zhao Wan,
Wang Wei,
Zhang Lili,
Cui Feng
Publication year - 2019
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5311
Subject(s) - biology , saliva , planthopper , virology , brown planthopper , vector (molecular biology) , virus , insect , delphacidae , transmission (telecommunications) , gene , homoptera , pest analysis , botany , genetics , hemiptera , biochemistry , engineering , electrical engineering , recombinant dna
BACKGROUND Persistent plant viruses transfer from insect gut to the hemolymph, and finally to the salivary glands before inoculation into the plant hosts with saliva during insect feeding. Virus accumulation in saliva is an important indicator for the transmission ability of an insect vector. In order to evaluate the transmission ability of the small brown planthopper to rice stripe virus (RSV), we successfully measured accumulation of RSV in the saliva of planthoppers via the absolute real‐time quantitative polymerase chain reaction method by quantifying the copy numbers of viral genes. RESULTS After feeding on an artificial diet for 24 h, the copy numbers of viral genes of capsid protein ( CP) and disease‐specific protein ( SP ) can be detected in the saliva collected from as few as ten viruliferous planthoppers and ten non‐viruliferous planthoppers after infected with RSV for 7 days. When the expression of planthopper G protein pathway suppressor 2 or c‐Jun N‐terminal kinase was knocked down, the copy numbers of CP and SP in the saliva varied accordingly. CONCLUSION Our study provided an accurate and convenient detection system to evaluate the transmission efficiency of RSV by small brown planthoppers, and this method may also be suitable for other persistent plant viruses. © 2019 Society of Chemical Industry