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Induced systemic resistance against Cucumber mosaic virus by Phoma sp. GS8‐2 stimulates transcription of pathogenesis‐related genes in Arabidopsis
Author(s) -
Elsharkawy Mohsen Mohamed
Publication year - 2019
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5193
Subject(s) - cucumber mosaic virus , biology , chitinase , arabidopsis , gene , cucumovirus , plant disease resistance , glucanase , pathogenesis , virus , microbiology and biotechnology , virology , plant virus , mutant , genetics , immunology
Background Cucumber mosaic virus (CMV) is a serious threat to vegetable production worldwide. The efficacy of Phoma sp. GS8‐2 was evaluated against CMV in Arabidopsis and cucumber plants. Results Arabidopsis and cucumber plants treated with barley grain inoculum (BGI) or cell‐free filtrate (CF) of GS8‐2 demonstrated decreased CMV severity and titre using enzyme‐linked immunosorbent assay relative to the control. Cucumber growth and yield parameters were significantly increased due to colonization with GS8‐2 under field conditions. Molecular mechanisms underlying mediated resistance induced by GS8‐2 against CMV were investigated. Real‐time polymerase chain reaction (RT‐PCR) results confirmed that both BGI and CF of GS8‐2 stimulated the transcription levels of pathogenesis related genes ( β1‐3 glucanase, chitinase, PR1, PAL1 and LOX1 ), which could be involved in induced resistance against CMV. Conclusion Exploring the expression of the highly upregulated genes in GS8‐2‐induced plants suggested the contribution of multiple plant defence pathways against CMV. © 2018 Society of Chemical Industry