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Paralogous Cyp51s mediate the differential sensitivity of Fusarium oxysporum to sterol demethylation inhibitors
Author(s) -
Zheng Bangxian,
Yan Leiyan,
Liang Wenxing,
Yang Qianqian
Publication year - 2019
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5127
Subject(s) - fusarium oxysporum , sterol , biology , mutant , fungicide , demethylation , gene , virulence , saccharomyces cerevisiae , microbiology and biotechnology , genetics , biochemistry , gene expression , cholesterol , botany , dna methylation
BACKGROUND As a soilborne fungus, Fusarium oxysporum can cause vascular wilt in numerous economically important crops. Application of antifungal drugs is the primary method for the control of F. oxysporum . Cyp51, a key enzyme of sterol biosynthesis is the main target of sterol demethylation inhibitors. RESULTS The F. oxysporum genome contains three paralogous CYP51 genes (named FoCYP51A , FoCYP51B and FoCYP51C ) that putatively encode sterol 14α‐demethylase enzymes. Each of the three genes was able to partially complement the Saccharomyces cerevisiae ERG11 mutant. Growth assays demonstrated that deletion mutants of FoCYP51B , but not FoCYP51A and FoCYP51C were significantly retarded in hyphal growth. Deletion of FoCYP51A (ΔFoCyp51A and ΔFoCyp51AC) led to increased sensitivity to 11 sterol demethylation inhibitors (DMIs). Interestingly, FoCYP51B deletion mutants (ΔFoCyp51B and ΔFoCyp51BC) exhibited significantly increased sensitivity to only four DMIs (two of which are in common with the 11 DMIs mentioned earlier). Deletion of FoCYP51C did not change DMI sensitivity of F. oxysporum . None of the three FoCYP51 s are involved in F. oxysporum virulence. The sensitivity of F. oxysporum isolates increased significantly when subjected to a mixture of different subgroups of DMIs classified based on the different sensitivities of FoCYP51 mutants to DMIs compared to the individual components. CONCLUSIONS FoCYP51A and FoCYP51B are responsible for sensitivity to different azoles. These findings have direct implications for fungicide application strategies of plant and human diseases caused by F. oxysporum . © 2018 Society of Chemical Industry