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Transcriptional response of bean bug ( Riptortus pedestris ) upon infection with entomopathogenic fungus, Beauveria bassiana JEF‐007
Author(s) -
Lee Se Jin,
Yang YiTing,
Kim Sihyeon,
Lee Mi Rong,
Kim Jong Cheol,
Park So Eun,
Hossain Muktadir S,
Shin Tae Young,
Nai YuShin,
Kim Jae Su
Publication year - 2019
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5117
Subject(s) - beauveria bassiana , biology , bassiana , entomopathogenic fungus , beauveria , microbiology and biotechnology , hemiptera , biological pest control , botany
Abstract BACKGROUND Entomopathogenic Beauveria bassiana has been used as a biocontrol agent for insect pests, but its effect at the molecular level on the hosts has not been studied in detail. Herein, we performed transcriptome analysis of bean bug, Riptortus pedestris (Hemiptera: Alydidae) in response to infection with a highly virulent strain of B . bassiana JEF‐007 ( Bb JEF‐007). RESULTS Based on RNA‐seq data from R. pedestris infected with Bb JEF‐007 compared with non‐infected bean bugs, infection was assumed to strongly activate (i) the energy production pathway by expressing dehydrogenases, (ii) metabolic pathways by expressing secreted proteins, GTPase, MBF2 transcription factor family, pigment‐dispersing factor, antioxidants, and cuticle proteins, and (iii) the immune response pathway by expressing serine‐threonine kinase in Toll pathway of bean bug. CONCLUSION We have established the platform for functional studies of the genes required for an immune response against entomopathogenic fungi like B. bassiana in the bean bug, R. pedestris . Moreover, this study also paves the way for genetic modification of B. bassiana to combat with the defense mechanism of R. pedestris . © 2018 Society of Chemical Industry