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Molecular identification of cultivable bacteria in the gut of adult Bactrocera tau (Walker) and their trapping effect
Author(s) -
Luo Mijuan,
Zhang Hehe,
Du Yinggang,
Idrees Atif,
He Longyan,
Chen Jiahua,
Ji Qing′e
Publication year - 2018
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5074
Subject(s) - biology , bacteria , enterobacter , microbiology and biotechnology , serratia , 16s ribosomal rna , escherichia coli , genetics , pseudomonas , gene
BACKGROUND It has been shown that some bacteria can attract their hosts. Our research aimed to identify cultivable bacterial isolates in the guts of sexually mature adult female and male Bactrocera tau and to evaluate their utility in trapping of B. tau . RESULTS Forty‐one strains of bacteria were isolated and identified from B. tau with morphological, physiological, biochemical and 16S rDNA analysis. The dominant bacterial genera shared by both females and males were Enterobacter , Providencia and Serratia . Thirteen bacterial isolates selected from female and male B. tau adults were cultured, and the attractiveness of bacterial fermented liquid and autoclaved supernatants from these strains to B. tau adults was tested. The laboratory test showed that both the autoclaved supernatants and fermented liquid could attract male and female B. tau , and the former was substantially more effective, with the autoclaved supernatants from all strains being significantly more attractive to adult B. tau . BF16, BF(12), BF23 and BF(32) were the most attractive bacteria to 8‐day‐old and sexually mature B. tau . Furthermore, the results of a subsequent field cage test showed that BF(12), BF23, and BF(32) were significantly more attractive to B. tau adults. CONCLUSION These results provide useful information for the development of bacterial biocontrol agents and their application as insecticides. © 2018 Society of Chemical Industry