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Alterations in the 5′ untranslated region of the 5‐enolpyruvylshikimate‐3‐phosphate synthase ( EPSPS ) gene influence EPSPS overexpression in glyphosate‐resistant Eleusine indica
Author(s) -
Zhang Chun,
Feng Li,
Tian Xingshan
Publication year - 2018
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.5042
Subject(s) - glyphosate , eleusine indica , atp synthase , gene , biology , three prime untranslated region , herbicide resistance , untranslated region , microbiology and biotechnology , genetics , botany , messenger rna , weed
BACKGROUND The herbicide glyphosate inhibits the enzyme 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS). Overexpression of the EPSPS gene is one of the molecular mechanisms conferring glyphosate resistance in weeds, but the transcriptional regulation of this gene is poorly understood. The EPSPS gene was found to be significantly up‐regulated following glyphosate treatment in a glyphosate‐resistant Eleusine indica population from southern China. To further investigate the regulation of EPSPS overexpression, the promoter of the EPSPS gene from this E. indica population was cloned and analyzed. RESULTS Two upstream regulatory sequences, Epro‐S (862 bp) and Epro‐R (877 bp), of EPSPS were obtained from glyphosate‐susceptible (S) and ‐resistant (R) E. indica plants, respectively, by high‐efficiency thermal asymmetric interlaced polymerase chain reaction (HiTAIL‐PCR). The Epro‐S and Epro‐R sequences were 99% homologous, except for two insertions (3 and12 bp) in the R sequence. The 12‐base insertion in the Epro‐R sequence was located in the 5′ untranslated region (UTR) pyrimidine nucleotide‐rich (Py‐rich) stretch element. Promoter activity tests showed that the 12‐base insertion resulted in significant enhancement of Epro‐R promoter activity, whereas the 3‐base insertion had little effect on Epro‐R promoter activity. CONCLUSION Alterations in the 5′ UTR Py‐rich stretch element of EPSPS are responsible for glyphosate‐induced EPSPS overexpression. Thus, EPSPS transcriptional regulation confers glyphosate resistance in this E. indica population. © 2018 Society of Chemical Industry