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Differential antioxidant enzyme activity in rapid‐response glyphosate‐resistant Ambrosia trifida
Author(s) -
Harre Nick T,
Nie Haozhen,
Jiang Yiwei,
Young Bryan G
Publication year - 2018
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4909
Subject(s) - glutathione reductase , paraquat , antioxidant , lipid peroxidation , biology , glyphosate , glutathione , glutathione peroxidase , juvenile , botany , oxidative stress , horticulture , biochemistry , enzyme , catalase , agronomy , ecology
BACKGROUND The giant ragweed ( Ambrosia trifida L.) rapid‐response (RR) biotype exhibits a sacrificial form of glyphosate resistance whereby an oxidative burst in mature leaves results in foliage loss, while juvenile leaves remain uninjured. This work investigated the safening capacity of antioxidant enzymes in RR juvenile leaves following glyphosate treatment and examined cross tolerance to paraquat. RESULTS Basal antioxidant enzyme activities were similar between glyphosate‐susceptible (GS) and RR biotypes. Lipid peroxidation was first detected in RR mature leaves at 8 h after treatment (HAT) and by 32 HAT was 5.3 and 21.1 times greater than that in RR juvenile leaves and GS leaves, respectively. Preceding lipid peroxidation in the RR biotype at 2 and 4 HAT, the only increase in enzymatic activity was observed in ascorbate‐glutathione cycle enzymes in RR juvenile leaves, particularly ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase. Sensitivity to paraquat was similar between biotypes. CONCLUSION The RR biotype is not inherently more tolerant to oxidative stress. The difference in tissue damage between RR juvenile and mature leaves following glyphosate treatment is attributable at least partially to the transient increase in antioxidant enzyme expression in juvenile leaves (0–8 HAT), but may also be attributable to lower overall RR induction in juvenile leaves compared with mature leaves. © 2018 Society of Chemical Industry