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PCR‐based detection of resistance to acetyl‐CoA carboxylase‐inhibiting herbicides in black‐grass ( Alopecurus myosuroides Huds) and ryegrass ( Lolium rigidum Gaud)
Author(s) -
Délye Christophe,
Matéjicek Annick,
Gasquez Jacques
Publication year - 2002
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.485
Subject(s) - lolium rigidum , biology , lolium , acetyl coa carboxylase , leucine , agronomy , population , allele , herbicide resistance , weed , gene , botany , pyruvate carboxylase , poaceae , genetics , biochemistry , amino acid , enzyme , demography , sociology
A simple method based upon allele‐specific PCR was developed to detect an isoleucine‐leucine substitution in the gene encoding chloroplastic acetyl‐coenzyme A carboxylase (ACCase) in two gramineous weeds: Lolium rigidum Gaud and Alopecurus myosuroides Huds. Analysis of 1800 A myosuroides and 750 L rigidum seedlings showed that the presence of ACCase leucine allele(s) conferred cross‐resistance to the cyclohexanedione herbicide cycloxydim and to the aryloxyphenoxypropionate herbicides fenoxaprop‐P‐ethyl and diclofop‐methyl. Seedlings containing ACCase leucine allele(s) could be either sensitive or resistant to the aryloxyphenoxypropionate herbicides haloxyfop‐P‐methyl and clodinafop‐propargyl. Successful detection of resistant plants in a field population of A myosuroides was achieved using this PCR assay. Using it with basic molecular biology laboratory equipment, the presence of resistant leucine ACCase allele(s) can be detected within one working day. © 2002 Society of Chemical Industry