Premium
Protein identification before and after glyphosate exposure in Lolium multiflorum genotypes
Author(s) -
Barroso Arthur AM,
de S Costa Marilia G,
Neto Nelson J,
dos Santos Juciléia I,
Balbuena Tiago S,
Carbonari Caio A,
Alves Pedro LCA
Publication year - 2018
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4831
Subject(s) - glyphosate , biology , abiotic component , lolium multiflorum , pesticide resistance , herbicide resistance , resistance (ecology) , chromosomal translocation , agronomy , lolium , crop , genetically modified crops , pesticide , weed , poaceae , transgene , genetics , gene , ecology
BACKGROUND Weeds reduce crop yields, and among the methods used to control these plants, the use of chemicals is preferred. However, the repeated application of herbicides with the same mechanism of action selects for resistant populations. The aim of this study was to evaluate glyphosate resistance in Lolium multiflorum (Lam.) and relate the resistance to protein expression in the absence and presence of the herbicide using a metabolic‐proteomic approach. RESULTS Glyphosate resistance was confirmed, with a sevenfold difference in resistance between susceptible and resistant genotypes. Among the possible mechanisms affecting resistance, mutations in the enzyme 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS), herbicide differential translocation and overexpression of EPSPS are suggested. Susceptible plants had higher growth than did resistant plants in the absence of the herbicide, in addition to greater expression of protein groups related to photosynthesis and to tolerance to biotic and abiotic stresses. With application of glyphosate, resistant plants maintained their metabolism and began to express EPSPS and other candidate proteins related to herbicide resistance. CONCLUSIONS In the absence of glyphosate, the susceptible plants would replace the resistant plants over time, and abiotic or biotic stresses would accelerate this process. Resistance in plants resulted from a combination of target‐site and non‐target‐site resistance mechanisms. We identified several candidate proteins that could be investigated in future studies on glyphosate resistance. © 2017 Society of Chemical Industry