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Overexpression of cytochrome P450 CYP6BG1 may contribute to chlorantraniliprole resistance in Plutella xylostella (L.)
Author(s) -
Li Xiuxia,
Li Ran,
Zhu Bin,
Gao Xiwu,
Liang Pei
Publication year - 2018
Publication title -
pest management science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.296
H-Index - 125
eISSN - 1526-4998
pISSN - 1526-498X
DOI - 10.1002/ps.4816
Subject(s) - plutella , diamondback moth , biology , cytochrome p450 , insecticide resistance , gene knockdown , knockdown resistance , transgene , rna interference , drosophila melanogaster , carboxylesterase , population , plutellidae , toxicity , toxicology , gene , enzyme , malathion , rna , genetics , pesticide , botany , biochemistry , lepidoptera genitalia , agronomy , medicine , environmental health , bendiocarb
BACKGROUND The diamondback moth Plutella xylostella (L.) is the most widely distributed pest of cruciferous crops and has developed resistance to most commonly used insecticides, including chlorantraniliprole. Resistance to chlorantraniliprole is likely caused by mutations of the target, the ryanodine receptor, and/or mediated by an increase in detoxification enzyme activities. Although target‐site resistance is documented in detail, resistance mediated by increased metabolism has rarely been reported. RESULTS The activity of cytochrome P450 was significantly higher in two resistant P. xylostella populations than in a susceptible one. Among ten detected cytochrome P450 genes, CYP6BG1 was significantly overexpressed (over 80‐fold) in a field‐resistant population compared with expression in a susceptible one. Knockdown of CYP6BG1 by RNA interference dramatically reduced the 7‐ethoxycoumarin‐O‐deethylase (7‐ECOD) activity of P450 by 45.5% and increased the toxicity of chlorantraniliprole toward P. xylostella by 26.8% at 48 h postinjection of double‐stranded RNA. By contrast, overexpression of CYP6BG1 in a transgenic Drosophila melanogaster line significantly decreased the toxicity of the insecticide to the transgenic flies. CONCLUSIONS Overexpression of CYP6BG1 may contribute to chlorantraniliprole resistance in P. xylostella . Our findings will provide new insights into the mechanisms of resistance to diamide insecticides in other insect pests. © 2017 Society of Chemical Industry